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second strand cdna synthesis kit  (Thermo Fisher)


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    Thermo Fisher second strand cdna synthesis kit
    Second Strand Cdna Synthesis Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/second+strand+cdna+synthesis+kit/revertaid+first+strand+cdna+synthesis+kit/pm40573407-60-16-21
    Average 90 stars, based on 1 article reviews
    second strand cdna synthesis kit - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Synthesized:

    Article Title: First description of a mobatvirus ( Hantaviridae ) in the Amazon region
    Article Snippet: RNA extraction was performed with the PureLink® RNA Mini Kit (Invitrogen) in accordance with the manufacturer's protocol. .. The first and second strands of complementary DNA (cDNA) were synthesized via the SuperScriptTM IV VILOTM MasterMix (Invitrogen) and the Second Strand cDNA Synthesis Kit (Invitrogen), respectively. .. The cDNA library was prepared via the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA).

    Article Title: Mining the secreted and membrane transcriptome of Hyalomma dromedarii ticks for identification of potential protective antigens
    Article Snippet: RNA was extracted from different tick life stages using TRIzol reagent (Invitrogen, Thermo Fisher Scientific), and the quality of the RNA was examined by electrophoresis, which showed no degradation of the RNA. mRNA was isolated using the Dynabeads Oligo(dT) 25 mRNA purification kit (Thermo Fisher Scientific). .. The first-strand cDNA was synthesized using the Invitrogen SuperScript III Reverse Transcriptase kit; The second-strand cDNA was synthesized using the E. coli RNase H, E. coli DNA polymerase and E. coli DNA ligase following the reaction protocol of the Second Strand cDNA Synthesis Kit (A48570; Invitrogen, Thermo Fisher Scientific). ..

    cDNA Synthesis:

    Article Title: First description of a mobatvirus ( Hantaviridae ) in the Amazon region
    Article Snippet: RNA extraction was performed with the PureLink® RNA Mini Kit (Invitrogen) in accordance with the manufacturer's protocol. .. The first and second strands of complementary DNA (cDNA) were synthesized via the SuperScriptTM IV VILOTM MasterMix (Invitrogen) and the Second Strand cDNA Synthesis Kit (Invitrogen), respectively. .. The cDNA library was prepared via the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA, USA).

    Article Title: Mining the secreted and membrane transcriptome of Hyalomma dromedarii ticks for identification of potential protective antigens
    Article Snippet: RNA was extracted from different tick life stages using TRIzol reagent (Invitrogen, Thermo Fisher Scientific), and the quality of the RNA was examined by electrophoresis, which showed no degradation of the RNA. mRNA was isolated using the Dynabeads Oligo(dT) 25 mRNA purification kit (Thermo Fisher Scientific). .. The first-strand cDNA was synthesized using the Invitrogen SuperScript III Reverse Transcriptase kit; The second-strand cDNA was synthesized using the E. coli RNase H, E. coli DNA polymerase and E. coli DNA ligase following the reaction protocol of the Second Strand cDNA Synthesis Kit (A48570; Invitrogen, Thermo Fisher Scientific). ..

    Article Title: Case Series of Adverse Pregnancy Outcomes Associated with Oropouche Virus Infection
    Article Snippet: RNA quantification was then conducted with the Qubit RNA HS Assay Kit and Qubit 4.0 fluorometer (Thermo Fisher Scientific, Carlsbad, CA, USA). .. For cDNA synthesis, the SuperScriptTM VILOTM MasterMix Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) and the Second Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) were utilized, and the cDNA was subsequently purified using the PureLink ® PCR Purification Kit (Thermo Fisher Scientific Baltics UAB, Vilnius, Lithuania). cDNA quantification was carried out with the DNA HS Assay Kit (Illumina, Inc., San Diego, CA, USA) on the Qubit 4.0. .. Genomic library preparation adhered to the Nextera XT DNA Kit (Illumina, Inc., San Diego, CA, USA) guidelines and was sequenced on the NextSeq 500 platform (Illumina, Inc., San Diego, CA, USA) using paired-end sequencing methodology.

    Article Title: Environmental factors and potential probiotic lineages shape the active prokaryotic communities associated with healthy Penaeus stylirostris larvae and their rearing water
    Article Snippet: .. Finally, reverse transcription was completed using the Second Strand cDNA Synthesis kit (ThermoFisher). .. Obtained cDNAs were sent to MrDNA (Shallowwater, TX, United States) to amplify and sequence the V4 region of 16S rRNA gene using the 515F/806R primers (Caporaso et al. ).

    Article Title: Exploring the anti‑inflammatory activity of boron compounds through the miR‑21/PTEN/AKT pathway in cecal ligation and puncture‑induced sepsis
    Article Snippet: The extraction of total RNA from paraffin-embedded kidney and liver tissues was performed using the High Pure FFPET RNA Isolation Kit (Invitrogen; Thermo Fisher Scientific, Inc.), following the manufacturer's guidelines. .. Subsequently, cDNA synthesis was performed with the Second Strand cDNA Synthesis Kit (Invitrogen; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol, utilizing a consistent quantity of RNA from each sample. ..

    Article Title: Case Series of Adverse Pregnancy Outcomes Associated with Oropouche Virus Infection.
    Article Snippet: RNA quantification was then conducted with the Qubit RNA HS Assay Kit and Qubit 4.0 fluorometer (Thermo Fisher Scientific, Carlsbad, CA, USA). .. For cDNA synthesis, the SuperScriptTM VILOTM MasterMix Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) and the Second Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) were utilized, and the cDNA was subsequently purified using the PureLink® PCR Purification Kit (Thermo Fisher Scientific Baltics UAB, Vilnius, Lithuania). cDNA quantification was carried out with the DNA HS Assay Kit (Illumina, Inc., San Diego, CA, USA) on the Qubit 4.0. .. Genomic library preparation adhered to the Nextera XT DNA Kit (Illumina, Inc., San Diego, CA, USA) guidelines and was sequenced on the NextSeq 500 platform (Illumina, Inc., San Diego, CA, USA) using paired-end sequencing methodology.

    Article Title: Exploring the anti‑inflammatory activity of boron compounds through the miR‑21/PTEN/AKT pathway in cecal ligation and puncture‑induced sepsis.
    Article Snippet: The present study investigated the impact of boric acid (BA) and borax (BX) on markers of inflammation and modifications in miR‐21/PTEN/AKT pathway genes in the liver and kidney tissues of Sprague Dawley male rats with sepsis induced by cecal ligation and puncture (clP). a total of 60 male Sprague Dawley rats were randomly divided into 6 groups, each containing 10 animals as follows: Control, CLP (where the model was created), 20 mg/kg BX (CLP + BX1), 40 mg/kg BX (CLP + BX2), 20 mg/kg BA (CLP + BA1) and 40 mg/kg BA (CLP + BA2).. Liver and kidney tissues were analyzed for histopathological changes, immunopositivity for tumor necrosis factor‐α, interleukin (IL)‐6 and IL‐10, and gene expression of microRNA‐21 (miR‐21), phosphatase and tensin homolog (PTEN) and AKT.. Gene expression analysis in the liver tissues revealed a significant decrease in miR‐21, and a marked but not significant decrease in PTEN levels in the CLP group, while AKT expression was significantly increased in the CLP group, and was significantly decreased in CLP + BA1 group compared with in the CLP group.

    Reverse Transcription:

    Article Title: Mining the secreted and membrane transcriptome of Hyalomma dromedarii ticks for identification of potential protective antigens
    Article Snippet: RNA was extracted from different tick life stages using TRIzol reagent (Invitrogen, Thermo Fisher Scientific), and the quality of the RNA was examined by electrophoresis, which showed no degradation of the RNA. mRNA was isolated using the Dynabeads Oligo(dT) 25 mRNA purification kit (Thermo Fisher Scientific). .. The first-strand cDNA was synthesized using the Invitrogen SuperScript III Reverse Transcriptase kit; The second-strand cDNA was synthesized using the E. coli RNase H, E. coli DNA polymerase and E. coli DNA ligase following the reaction protocol of the Second Strand cDNA Synthesis Kit (A48570; Invitrogen, Thermo Fisher Scientific). ..

    Article Title: Environmental factors and potential probiotic lineages shape the active prokaryotic communities associated with healthy Penaeus stylirostris larvae and their rearing water
    Article Snippet: .. Finally, reverse transcription was completed using the Second Strand cDNA Synthesis kit (ThermoFisher). .. Obtained cDNAs were sent to MrDNA (Shallowwater, TX, United States) to amplify and sequence the V4 region of 16S rRNA gene using the 515F/806R primers (Caporaso et al. ).

    other:

    Article Title: Environmental factors and potential probiotic lineages shape the active prokaryotic communities associated with healthy Penaeus stylirostris larvae and their rearing water.
    Article Snippet: Finally, reverse transcription was completed using the Second Strand cDNA 209 Synthesis kit (ThermoFisher).

    Purification:

    Article Title: Case Series of Adverse Pregnancy Outcomes Associated with Oropouche Virus Infection
    Article Snippet: RNA quantification was then conducted with the Qubit RNA HS Assay Kit and Qubit 4.0 fluorometer (Thermo Fisher Scientific, Carlsbad, CA, USA). .. For cDNA synthesis, the SuperScriptTM VILOTM MasterMix Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) and the Second Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) were utilized, and the cDNA was subsequently purified using the PureLink ® PCR Purification Kit (Thermo Fisher Scientific Baltics UAB, Vilnius, Lithuania). cDNA quantification was carried out with the DNA HS Assay Kit (Illumina, Inc., San Diego, CA, USA) on the Qubit 4.0. .. Genomic library preparation adhered to the Nextera XT DNA Kit (Illumina, Inc., San Diego, CA, USA) guidelines and was sequenced on the NextSeq 500 platform (Illumina, Inc., San Diego, CA, USA) using paired-end sequencing methodology.

    Article Title: Case Series of Adverse Pregnancy Outcomes Associated with Oropouche Virus Infection.
    Article Snippet: RNA quantification was then conducted with the Qubit RNA HS Assay Kit and Qubit 4.0 fluorometer (Thermo Fisher Scientific, Carlsbad, CA, USA). .. For cDNA synthesis, the SuperScriptTM VILOTM MasterMix Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) and the Second Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) were utilized, and the cDNA was subsequently purified using the PureLink® PCR Purification Kit (Thermo Fisher Scientific Baltics UAB, Vilnius, Lithuania). cDNA quantification was carried out with the DNA HS Assay Kit (Illumina, Inc., San Diego, CA, USA) on the Qubit 4.0. .. Genomic library preparation adhered to the Nextera XT DNA Kit (Illumina, Inc., San Diego, CA, USA) guidelines and was sequenced on the NextSeq 500 platform (Illumina, Inc., San Diego, CA, USA) using paired-end sequencing methodology.

    Polymerase Chain Reaction:

    Article Title: Case Series of Adverse Pregnancy Outcomes Associated with Oropouche Virus Infection
    Article Snippet: RNA quantification was then conducted with the Qubit RNA HS Assay Kit and Qubit 4.0 fluorometer (Thermo Fisher Scientific, Carlsbad, CA, USA). .. For cDNA synthesis, the SuperScriptTM VILOTM MasterMix Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) and the Second Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) were utilized, and the cDNA was subsequently purified using the PureLink ® PCR Purification Kit (Thermo Fisher Scientific Baltics UAB, Vilnius, Lithuania). cDNA quantification was carried out with the DNA HS Assay Kit (Illumina, Inc., San Diego, CA, USA) on the Qubit 4.0. .. Genomic library preparation adhered to the Nextera XT DNA Kit (Illumina, Inc., San Diego, CA, USA) guidelines and was sequenced on the NextSeq 500 platform (Illumina, Inc., San Diego, CA, USA) using paired-end sequencing methodology.

    Article Title: Case Series of Adverse Pregnancy Outcomes Associated with Oropouche Virus Infection.
    Article Snippet: RNA quantification was then conducted with the Qubit RNA HS Assay Kit and Qubit 4.0 fluorometer (Thermo Fisher Scientific, Carlsbad, CA, USA). .. For cDNA synthesis, the SuperScriptTM VILOTM MasterMix Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) and the Second Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) were utilized, and the cDNA was subsequently purified using the PureLink® PCR Purification Kit (Thermo Fisher Scientific Baltics UAB, Vilnius, Lithuania). cDNA quantification was carried out with the DNA HS Assay Kit (Illumina, Inc., San Diego, CA, USA) on the Qubit 4.0. .. Genomic library preparation adhered to the Nextera XT DNA Kit (Illumina, Inc., San Diego, CA, USA) guidelines and was sequenced on the NextSeq 500 platform (Illumina, Inc., San Diego, CA, USA) using paired-end sequencing methodology.

    DNA HS Assay:

    Article Title: Case Series of Adverse Pregnancy Outcomes Associated with Oropouche Virus Infection
    Article Snippet: RNA quantification was then conducted with the Qubit RNA HS Assay Kit and Qubit 4.0 fluorometer (Thermo Fisher Scientific, Carlsbad, CA, USA). .. For cDNA synthesis, the SuperScriptTM VILOTM MasterMix Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) and the Second Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) were utilized, and the cDNA was subsequently purified using the PureLink ® PCR Purification Kit (Thermo Fisher Scientific Baltics UAB, Vilnius, Lithuania). cDNA quantification was carried out with the DNA HS Assay Kit (Illumina, Inc., San Diego, CA, USA) on the Qubit 4.0. .. Genomic library preparation adhered to the Nextera XT DNA Kit (Illumina, Inc., San Diego, CA, USA) guidelines and was sequenced on the NextSeq 500 platform (Illumina, Inc., San Diego, CA, USA) using paired-end sequencing methodology.

    Article Title: Case Series of Adverse Pregnancy Outcomes Associated with Oropouche Virus Infection.
    Article Snippet: RNA quantification was then conducted with the Qubit RNA HS Assay Kit and Qubit 4.0 fluorometer (Thermo Fisher Scientific, Carlsbad, CA, USA). .. For cDNA synthesis, the SuperScriptTM VILOTM MasterMix Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) and the Second Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Carlsbad, CA, USA) were utilized, and the cDNA was subsequently purified using the PureLink® PCR Purification Kit (Thermo Fisher Scientific Baltics UAB, Vilnius, Lithuania). cDNA quantification was carried out with the DNA HS Assay Kit (Illumina, Inc., San Diego, CA, USA) on the Qubit 4.0. .. Genomic library preparation adhered to the Nextera XT DNA Kit (Illumina, Inc., San Diego, CA, USA) guidelines and was sequenced on the NextSeq 500 platform (Illumina, Inc., San Diego, CA, USA) using paired-end sequencing methodology.



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